Using primary sources (Links to an external site.), compare and contrast the experiences of at least 2 of the Thirteen original colonies. In your comparison, find and review the original charter of each colony (see Avalon (Links to an external site.) and Library of Congress (Links to an external site.)), paying attention to its stated purpose and objectives. How are the charters similar or different? For instance, you might compare Plymouth and Jamestown, or Georgia and Rhode Island.
When comparing the two charters (and colonial experience based on these two charters), consider: how the charter helped determine who had power (and who did not), how the charter influenced the growth of the colony, and how it impacted interactions with the Native Americans? What can we learn from these similarities and/or differences?
Note: A primary source is a document or artifact created at the time the event took place (such as the charters themselves). Your answer should use the charters, but can also use other primary sources (optional).
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Mixed Unknown Activity / Experiment Directions: The mixed unknown exercise has been modified for the online experience. You will be receiving PowerPoint images for “Day 1” of your unknown. These images include the Gram stains for both organisms, and isolated colonies on MacConkey’s (Gram-) and Columbia CNA (Gram+) agars. We have created individual Discussion Board threads in your Blackboard site in which you will be able to find your PowerPoint images. The ultimate goal of this experiment is to determine the identity of the two bacterial species that you have been given, and writing them in proper binomial nomenclature. Please find the Mixed Unknown Worksheet below. On the day that the assignment window begins, you will be given your images. IN ORDER TO MAKE YOUR FINAL SPECIES IDENTIFICATION, YOU WILL NEED TO USE THE INFORMATION IN THOSE IMAGES TO ORDER ADDITIONAL TESTS. You will get two media test request deadlines that you can request a total of six (6) tests for each organism—for a total of twelve (12). By the first deadline, you should order a few tests that you believe are indicated for your organism(s). Make sure to indicate if you are ordering the test for your Gram+ or Gram – organism. Your instructor will respond with your results for those tests, if indicated. Once you analyze the first set of tests, you may have one additional set of media requests that you believe will narrow down your specific organism. IF YOU DO NOT ORDER MEDIA BY THE FIRST DEADLINE, YOU WILL ONLY GET ONE CHANCE TO ORDER MEDIA. When ordering media, you MUST use the correct name of the test requested (ex.: order ‘Skim Milk Agar’ test, not ‘casein’, order ‘Starch Agar’, not ‘amylase’, etc.). You will need to have all of the information on this sheet filled out before uploading it to your discussion board thread that has been created for you. Any portion not filled out when you turn it in, will result in no points for that section. You must include the genus and species of your organism, spelled out completely, in proper binomial nomenclature, to receive full credit. YOU MUST CORRECTLY IDENTIFY THE GENUS AND SPECIES OF BOTH ORGANISMS IN ORDER TO RECEIVE FULL CREDIT. Since MacConkey’s and CNA are already provided, you do not need to order them in your ‘TEST MEDIA REQUESTED” section of the table, but you should interpret the results in the second table of your mixed unknown identification form. For tests that require several results (ex.: IMVIC, carbohydrate fermentation, etc.), please provide the results in such a way that they are easy to understand. For example:
• +/+/-/- could be a way to write your IMVIC results • G: A/+, S: -/-, L: A/- could be a way to write your carbohydrate fermentation results
MIXED UNKNOWN IDENTIFICATION FORM Print Name: Lab Section: Signature: Unknown Specimen Number: (-5 if left blank or the incorrect number. This is your patient ID. Neglecting to label or mislabeling results in patient death s.)
REQUESTING TEST MEDIA
DO NOT TAKE MEDIA FROM MEDIA BENCH WITHOUT PERMISSION FROM YOUR INSTRUCTOR. Reagents & hydrogen peroxide (if requesting catalase test) DO NOT have to be requested. Before coming to the Media Bench:
1. Fill each test media in the correct column in the correct order starting from #1. Use an ink pen. 2. Prepare all your labels. 3. Bring a test tube rack, if you are requesting tube media. 4. Bring these to the Media Bench where your instructor will provide you with the requested media.
TEST MEDIA REQUESTED
For Gram-Positive Organism For Gram-Negative Organism #1 #1 #2 #2 #3 #3 #4 #4 #5 #5 #6 #6
UNIQUE CHARACTERISTICS & TEST RESULTS points For Gram-Positive Organism points For Gram-Negative Organism Gram Stain Reaction: Gram Stain Reaction:
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